Basics
- Mammalian limb structure: All mammals share a common five-digit limb blueprint (pentadactyl limb).
- Bats’ uniqueness: Only mammals capable of true powered flight, achieved via wings.
- Wing formation: Forelimbs elongate digits 2–5; thin skin sheet called chiropatagium stretches between them.
Relevance :
- GS3 – Science & Technology / Biotechnology
- Evolutionary biology, genetics, regulatory evolution.
- Developmental biology insights applicable to medicine (congenital limb disorders, syndactyly).
- Comparative genomics and single-cell analysis techniques.

Scientific Question
- How do bats develop wings while sharing the same genes as other mammals?
- Early embryos of bats, mice, dolphins, etc., look almost identical.
- Key concept: Regulatory evolution — altering when, where, and how genes are activated, rather than changing gene sequences.
Chiropatagium Mystery
- Traditionally, mammals lose skin between fingers via apoptosis (programmed cell death).
- Hypothesis: Bats suppressed interdigital apoptosis → wing formation.
- New study challenges this: apoptosis still occurs in bat wing tissue; something else must sustain the chiropatagium.
Study Approach
- Species used: Bats (Carollia perspicillata) and mice.
- Methodology:
- Single-cell RNA sequencing of >180,000 embryonic limb cells.
- Created an interspecies limb atlas for developmental comparison.
- Computational modelling to identify cell types and gene activity.
Key Findings
- Cell-level similarity: Bat and mouse limbs have almost identical cell types; no novel cell type was invented.
- Specialized fibroblasts:
- A population of connective tissue cells (fibroblasts) is repositioned between digits in bats.
- These fibroblasts express transcription factors MEIS2 and TBX3, switched off in other mammals at this stage.
- Evolutionary co-option:
- Existing gene programs redeployed in a new context → new structures without new genes.
- Functional validation in mice:
- Transgenic mice expressing bat MEIS2 and TBX3 in distal limbs → fused, webbed digits resembling early bat wings.
- Shows regulatory changes alone can drive structural innovation.
Mechanistic Insights
- Regulatory shifts: Key to bat wing evolution; small changes in gene timing/location produce dramatic morphological differences.
- Apoptosis still occurs: Wing webbing persists due to specialized fibroblasts, not apoptosis suppression.
- Transcription factors as “genetic dials”: MEIS2 and TBX3 are sufficient to partially activate wing-building programs.
Broader Implications
- Evolutionary biology:
- Supports the concept that diverse limb morphologies (bat wings, bird wings, whale flippers, fish fins) arise from modifying universal developmental blueprints.
- Developmental biology & medicine:
- May inform understanding of syndactyly (fused digits in humans).
- Insights into gene regulation during limb formation could aid diagnosis and treatment of congenital limb disorders.
- Research tools: Single-cell RNA sequencing and cross-species analysis enable mapping of regulatory changes driving evolution.
Takeaways
- Bat wings evolved through regulatory evolution, not new genes.
- Existing cell types were repurposed and strategically deployed.
- Small genetic shifts can yield major morphological innovations.
- Study demonstrates the power of transcription factors in shaping limbs across species.
- Highlights the potential of comparative genomics and single-cell analysis in unraveling evolutionary mechanisms.